Total submissions: 1
Submitter | RCV | SCV | Clinical significance | Condition | Last evaluated | Review status | Method | Comment |
---|---|---|---|---|---|---|---|---|
Institute of Reproductive and Stem Cell Engineering, |
RCV000660891 | SCV000680460 | pathogenic | 3-Oxo-5 alpha-steroid delta 4-dehydrogenase deficiency | 2018-02-01 | criteria provided, single submitter | clinical testing | The novel mutation p.G196R and a known mutation p.Q6X were identified in a patient who had perineal hypospadias. Although in vitro studies have not been performed to investigate the functional impact of the p.G196R mutation, several lines of evidence suggest that it is a disease-causing mutation. Firstly, codon 196 is highly conserved. Secondly, in silico analysis by the PolyPhen-2 and PROVEAN software predicted p.G196R to be a "probably damaging" and "deleterious" mutation, respectively. Finally, in this same codon, pathogenic mutations G196S and G196D have been identified previously. Experiments have shown that the G196S mutation could reduce the overall enzyme activity and increase the optimum pH slightly. According to the interpretation of the American College of Medical Genetics and Genomics (ACMG) guidelines, the missense mutation p.G196R was likely to be pathogenic. |