Total submissions: 3
Submitter | RCV | SCV | Clinical significance | Condition | Last evaluated | Review status | Method | Comment |
---|---|---|---|---|---|---|---|---|
Ambry Genetics | RCV000561132 | SCV000667207 | pathogenic | Hereditary cancer-predisposing syndrome | 2024-07-03 | criteria provided, single submitter | clinical testing | The p.V157D pathogenic mutation (also known as c.470T>A), located in coding exon 4 of the TP53 gene, results from a T to A substitution at nucleotide position 470. The valine at codon 157 is replaced by aspartic acid, an amino acid with highly dissimilar properties. This variant has also reported in a tumor and the germline of a patient with lung cancer at age 22, and a family history of multiple early-onset cancers; functional analysis of p.V157D showed impaired p51 transactivation ability (Wang Z et al. Cancer Lett. 2014 Jan;342:36-42). Studies conducted in human cell lines indicate this alteration is deficient at growth suppression (Kotler E et al. Mol. Cell 2018 Jul;71:178-190.e8; Giacomelli AO et al. Nat. Genet. 2018 Oct;50:1381-1387). Furthermore, alterations at the same location (p.V157A, p.V157F) have been reported in families meeting Li-Fraumeni-like syndrome criteria are anticipated to result in a significant decrease in structural stability of the DNA-binding domain (Cho Y et al. Science. 1994 Jul; 265(5170):346-55; Kitayner et al. Nat. Struct. Mol. Biol. 2010 Apr;17(4):423-9; Calhoun S et al. Biochemistry. 2011 Jun;50:5345-53). This amino acid position is not well conserved in available vertebrate species. In addition, this alteration is predicted to be deleterious by in silico analysis. Based on the supporting evidence, this alteration is interpreted as a disease-causing mutation. |
Labcorp Genetics |
RCV000807881 | SCV000947957 | uncertain significance | Li-Fraumeni syndrome | 2022-10-25 | criteria provided, single submitter | clinical testing | This sequence change replaces valine, which is neutral and non-polar, with aspartic acid, which is acidic and polar, at codon 157 of the TP53 protein (p.Val157Asp). This variant is not present in population databases (gnomAD no frequency). This missense change has been observed in individual(s) with clinical features of Li-Fraumeni syndrome (PMID: 23981578, 32994724). ClinVar contains an entry for this variant (Variation ID: 482231). Advanced modeling performed at Invitae incorporating data from internal and/or published experimental studies (PMID: 12826609, 29979965, 30224644) indicates that this missense variant is expected to disrupt TP53 function. Experimental studies have shown that this missense change affects TP53 function (PMID: 12826609, 23981578, 29979965, 30224644). In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance. |
Myriad Genetics, |
RCV004024481 | SCV004932755 | likely pathogenic | Li-Fraumeni syndrome 1 | 2024-02-14 | criteria provided, single submitter | clinical testing | This variant is considered likely pathogenic. Functional studies indicate this variant impacts protein function [PMID: 29979965]. This variant is expected to disrupt protein structure [Myriad internal data]. |