Total submissions: 3
Submitter | RCV | SCV | Clinical significance | Condition | Last evaluated | Review status | Method | Comment |
---|---|---|---|---|---|---|---|---|
Counsyl | RCV000673439 | SCV000798641 | likely pathogenic | Nemaline myopathy 2 | 2018-03-16 | criteria provided, single submitter | clinical testing | |
Labcorp Genetics |
RCV000673439 | SCV002266037 | likely pathogenic | Nemaline myopathy 2 | 2023-01-20 | criteria provided, single submitter | clinical testing | In summary, the currently available evidence indicates that the variant is pathogenic, but additional data are needed to prove that conclusively. Therefore, this variant has been classified as Likely Pathogenic. Algorithms developed to predict the effect of sequence changes on RNA splicing suggest that this variant may disrupt the consensus splice site. ClinVar contains an entry for this variant (Variation ID: 557313). This variant has not been reported in the literature in individuals affected with NEB-related conditions. This variant is present in population databases (no rsID available, gnomAD 0.007%). This sequence change affects a donor splice site in intron 137 of the NEB gene. It is expected to disrupt RNA splicing. Variants that disrupt the donor or acceptor splice site typically lead to a loss of protein function (PMID: 16199547), and loss-of-function variants in NEB are known to be pathogenic (PMID: 25205138). |
Women's Health and Genetics/Laboratory Corporation of America, |
RCV002271561 | SCV002555757 | likely pathogenic | Nemaline myopathy | 2024-09-19 | criteria provided, single submitter | clinical testing | Variant summary: NEB c.20787+2T>C is located in a canonical splice-site and is predicted to affect mRNA splicing resulting in a significantly altered protein due to either exon skipping, shortening, or inclusion of intronic material. Variants that disrupt the consensus splice site are a relatively common cause of aberrant splicing and loss of NEB function. Several computational tools predict a significant impact on normal splicing: Three predict the variant abolishes a 5 splicing donor site. However, these predictions have yet to be confirmed by functional studies. The variant was absent in 248568 control chromosomes. To our knowledge, no occurrence of c.20787+2T>C in individuals affected with Nemaline Myopathy 2 and no experimental evidence demonstrating its impact on protein function have been reported. ClinVar contains an entry for this variant (Variation ID: 557313). Based on the evidence outlined above, the variant was classified as likely pathogenic. |