Total submissions: 4
Submitter | RCV | SCV | Clinical significance | Condition | Last evaluated | Review status | Method | Comment |
---|---|---|---|---|---|---|---|---|
Genomic Medicine Lab, |
RCV000240867 | SCV001547493 | likely pathogenic | Bardet-Biedl syndrome 22 | 2020-05-14 | criteria provided, single submitter | clinical testing | The IFT74 gene encodes an intraflagellar transport protein that is involved in transport of ciliary proteins along the axonemal microtubules. Variants in this gene are associated with Bardet-Biedl syndrome 20 (MIM#617119), an autosomal recessive disorder characterized by retinitis pigmentosa, obesity, polydactyly, hypogonadism, and intellectual disability. This specific splice site variant has been described once before (PMID: 27486776). There is a Clinvar entry for this variant (Variant ID: 254276). This variant is present in population databases, with an allele frequency of 0.0021% in gnomAD (5 heterozygotes). |
Invitae | RCV002518558 | SCV003294354 | uncertain significance | not provided | 2022-07-13 | criteria provided, single submitter | clinical testing | This sequence change affects an acceptor splice site in intron 19 of the IFT74 gene. While this variant is not anticipated to result in nonsense mediated decay, it likely alters RNA splicing and results in a disrupted protein product. This variant is present in population databases (rs200699377, gnomAD 0.005%). Disruption of this splice site has been observed in individual(s) with Bardet-Biedl syndrome (PMID: 27486776, 32144365, 33748949). This variant is also known as c.16850–1G>T. ClinVar contains an entry for this variant (Variation ID: 254276). Variants that disrupt the consensus splice site are a relatively common cause of aberrant splicing (PMID: 17576681, 9536098). Algorithms developed to predict the effect of sequence changes on RNA splicing suggest that this variant may disrupt the consensus splice site. In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance. |
Prevention |
RCV003409371 | SCV004113348 | likely pathogenic | IFT74-related disorder | 2022-11-23 | criteria provided, single submitter | clinical testing | The IFT74 c.1685-1G>T variant is predicted to disrupt the AG splice acceptor site and interfere with normal splicing. This variant has been previously reported in the homozygous or compound heterozygous state in individuals with Bardet-Biedl syndrome (Lindstrand et al. 2016. PubMed ID: 27486776; Kleinendorst et al. 2020. PubMed ID: 32144365; Mardy et al. 2021. PubMed ID: 33748949). This variant is reported in 0.0048% of alleles in individuals of European (Non-Finnish) descent in gnomAD (http://gnomad.broadinstitute.org/variant/9-27062615-G-T). Variants that disrupt the consensus splice acceptor site in IFT74 are expected to be pathogenic. This variant is interpreted as likely pathogenic. |
OMIM | RCV000240867 | SCV000299366 | pathogenic | Bardet-Biedl syndrome 22 | 2021-10-21 | no assertion criteria provided | literature only |