Total submissions: 3
Submitter | RCV | SCV | Clinical significance | Condition | Last evaluated | Review status | Method | Comment |
---|---|---|---|---|---|---|---|---|
Gene |
RCV000627441 | SCV000748440 | likely pathogenic | not provided | 2018-04-03 | criteria provided, single submitter | clinical testing | The c.1144_1145delCT variant in the LIG4 gene has been reported previously using alternate nomenclature (c.1142-1143delCT) on the opposite allele (in trans) with another LIG4 variant in an individual with autoimmune hemolytic anemia, severe pneumonia, thrombocytopenia, microcephaly, and growth retardation (Jiang et al., 2016). The c.1144_1145delCT variant causes a frameshift starting with codon Leucine 382, changes this amino acid to a Glutamic acid residue, and creates a premature Stop codon at position 5 of the new reading frame, denoted p.Leu382GlufsX5. This variant is predicted to cause loss of normal protein function through protein truncation. The c.1144_1145delCT variant is not observed at a significant frequency in large population cohorts (Lek et al., 2016). We interpret c.1144_1145delCT as a likely pathogenic variant. |
Labcorp Genetics |
RCV001860490 | SCV002246100 | pathogenic | DNA ligase IV deficiency | 2024-03-12 | criteria provided, single submitter | clinical testing | This sequence change creates a premature translational stop signal (p.Leu382Glufs*5) in the LIG4 gene. While this is not anticipated to result in nonsense mediated decay, it is expected to disrupt the last 530 amino acid(s) of the LIG4 protein. This variant is present in population databases (no rsID available, gnomAD 0.006%). This premature translational stop signal has been observed in individual(s) with ligase IV syndrome (PMID: 26762768). ClinVar contains an entry for this variant (Variation ID: 523952). This variant disrupts a region of the LIG4 protein in which other variant(s) (p.Arg814*) have been determined to be pathogenic (PMID: 11779494, 16088910, 25239263, 27063650). This suggests that this is a clinically significant region of the protein, and that variants that disrupt it are likely to be disease-causing. For these reasons, this variant has been classified as Pathogenic. |
Juno Genomics, |
RCV001860490 | SCV005416590 | pathogenic | DNA ligase IV deficiency | criteria provided, single submitter | clinical testing | PVS1_Strong+PM2_Supporting+PM3_Strong+PP4 |